Figure 6.
Suppression of the atp10 null mutant by ATP23. (A) Growth of the atp10 mutant and transformants on rich glycerol/ethanol. The wild-type strain W303-1A, the atp10 null mutant W303ΔATP10 (ΔATP10), and the null mutant transformed either with ATP10 in an episomal plasmid [(ΔATP10 + ATP10 (e)] or with ATP23 on and episomal [ΔATP10 + ATP23(e)] or integrative plasmid [(ΔATP10 + ATP23(i)] were grown in liquid YPD. Serial dilutions were spotted on YPD and on YEPG medium and incubated at 30°C for 2 d. (B) The same strains (except for the atp10 mutant with ATP23 gene on an episomal plasmid) were labeled in vivo with [35S]methionine in the presence of cycloheximide as described in the legend to Figure 3A. The position of mature subunit 6 (Atp6) is indicated by the arrow.