Table 2.
Respiratory and ATPase activities of mitochondria from wild-type and atp22 mutants
| Strain | % ρ+ | ATPase (μmol/min/mg) |
% Inh | |
|---|---|---|---|---|
| −Oligomcyin | +Oligomycin | |||
| Experiment 1 | ||||
| W303-1B | >99 | 5.28 ± 0.03 | 1.15 ± 0.03 | 77 |
| D273-10B/A1 | >99 | 5.65 ± 0.02 | 1.38 ± 0.03 | 76 |
| E884 | 35 | 1.80 ± 0.05 | 1.82 ± 0.08 | 0 |
| aE884/UL1 | 20 | 2.36 ± 0.01 | 2.41 ± 0.05 | 0 |
| W303ΔATP23 | 15 | 2.17 ± 0.07 | 2.22 ± 0.02 | 0 |
| W303ΔATP23/R1 | 76 | 2.26 ± 0.04 | 1.93 ± 0.01 | 15 |
| W303ΔATP23/R2 | 80 | 2.51 ± 0.05 | 1.96 ± 0.06 | 22 |
| W303ΔATP23 + ATP23(E→Q) (i) | 66 | 4.47 ± 0.34 | 0.33 ± 0.06 | 92.6 |
| W303ΔATP23 + ATP23(E→Q) (e) | 76 | 6.96 ± 0.43 | 1.39 ± 0.07 | 80 |
| Experiment 2 | ||||
| W303-1A | >99 | 4.70 | 0.42 | 91 |
| W303ΔATP10 | 37 | 1.90 | 1.78 | 6 |
| W303ΔATP10 + ATP23 (i) | 45 | 2.63 | 1.80 | 32 |
| W303ΔATP10 + ATP23 (e) | 47 | 1.80 | 1.05 | 42 |
Mitochondria were prepared from cells grown in YPGal. Samples of the cultures were used to test for the percentage of ρ−/o cells. ATPase activity was measured at 37°C. The values reported in experiment 1 are averages of duplicate assays with the ranges indicated.