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. 2007 Feb;18(2):581–593. doi: 10.1091/mbc.E06-07-0612

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© 2007 by The American Society for Cell Biology

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Figure 7. — Binding to PtdIns(3)P is not required for Atg27 function. (A) Atg27 localization is not affected by Vps34. Chromosomally tagged Atg20-GFP and Atg27-GFP strains expressing plasmid-based vps34^ts and RFP-Ape1 (WLY50 and WLY51, respectively) were grown in selective SMD medium to OD₆₀₀ = 0.8 at 26°C or shifted to 38°C for 12 min before imaging. (B) Atg14 does not affect Atg27 PAS localization. The Atg27-GFP atg14Δ strain (WLY52) expressing RFP-Ape1 was grown in selective SMD medium and visualized by fluorescence microscopy. Bar, 5 μm.