Table 1.
Primer sequences
Sizes | Positionsa | 5′-primers (5′→3′) | 3′-primers (5′→3′) | |
---|---|---|---|---|
Primers for RT-PCR | ||||
Fluc | 495 bp | ORF | AGAACTGCCTGCGTGAGATT | ATCCAGATCCACAACCTTCG |
Rluc | 273 bp | ORF | TCTTCGTGGAAACCATGTTG | TGGCACCTTCAACAATAGCA |
mTERT | 358 bp | ORF | CTAAAGGCCTCTGGCTCCTT | ATCTCCCATACCACCCATGA |
mCRR9 | 454 bp | ORF | TGCAGCCTTTCACCTTCTTT | GCCAAAGGCATAGACACCAT |
PCR primers for probes | ||||
CpG1 | 1.1 kb | −1080 to +31 bp | CGCCCCTTAGATCCAAACTT | CAGCGCTGCCTGAAACTC |
mHS1 | 387 bp | −2797 to −2411 bp | GGTAGGCCTTCCTCTGCTCT | TTTGGATTTTTGAGCCCTTG |
α-globin (Hba-a1) | 995 bp | −2380 to −1386 bp | AGTCCTGCTTGCAGTCCCTA | TGGGTTACAGTTTTGCACCA |
β-globin (Hbb-b1) | 447 bp | −1291 to −826 bp | CTTCCCAGGTCCTTCTCCTC | CCCAGTGAAGGGATCTTGAA |
Primers for ChIP | ||||
hTERT | 235 bp | −179 to +56 bp | CAGGACCGCGCTTCCCACG | GGCTTCCCACGTGCGCAGC |
hCRR9 | 234 bp | −463 to −230 bp | GACATGGCCCCCACTTG | CCCTGGGAAACGGAGTCT |
ORF, open reading frame.
a Relative to transcription start sites.