Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Substrate dependency of 2-HPCL. Lyase activity was measured at increasing concentrations of 2-hydroxy-3-methylhexadecanoyl-CoA on the partially purified enzyme preparation after phosphocellulose chromatography. The velocity (V, expressed in mU/ml) vs. substrate concentration (S, expressed in μM) curve was linearly transformed according to the method of Lineweaver-Burk as shown (Inset). An apparent Km of 15 μM could be calculated.
