Figure 5.

cDNA and deduced amino acid sequence of Hs 2-HPCL. The cDNA shown is based on overlapping sequences, determined at least once in both directions of the HindIII/NotI inserts of the Lafmid BA vector containing either T77417 or R17722 and of the 1.8-kb amplicon. Accession numbers of other ESTs overlapping this sequence are AA306411, AA156133, W74012, AA056397, AA298588, F08168, W21374, R58040, H10428, AA150650, AA643224, AI380322, and Z41242. The start codon and the corresponding methionine are indicated in bold. The position of the stop codon is marked by an asterisk. Sequences corresponding to the tryptic peptides, obtained from rat enzyme, are underlined. The C-terminal peroxisome targeting sequence [R]SNM is printed in bold, and the TPP-binding region is underlined twice.