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Subcellular localization of GFP fusion proteins in cultured mouse fibroblasts. Immortalized mouse PEX5+/− fibroblasts (a, c, and d) and PEX5−/− fibroblasts (b) were transfected with the construct coding for GFP fused to Hs 2-HPCL (a, b, and c) or to the C-terminal pentapeptide of the human lyase (d). After 26 h, cells were analyzed by fluorescence microscopy, either for direct fluorescence of GFP (a, b, and d) or, after immunostaining, for peroxisomal thiolase (c).
