Table 1.
Animal treatment | ||
---|---|---|
Parameter | Corn oil | TCDD |
H2O2 production | 57 ± 12 | 37 ± 18 |
O2 utilization | 324 ± 27 | 586 ± 45* |
RCR | 4.3 ± 0.4 | 3.9 ± 0.5 |
Membrane potential | 1.7 ± 0.2 | 1.6 ± 0.1 |
ATP production | 469 ± 37 | 281.3 ± 18* |
ATP/O ratio | 1.45 ± 0.15 | 0.48 ± 0.11* |
Hepatic ATP | 6.2 ± 0.4 | 3.4 ± 0.3* |
Liver mitochondria were prepared one week after treating male C57BL/6J mice with one dose of TCDD (5 μg/kg) or with corn oil vehicle. The units are: H2O2 production, pmol min−1 mg protein−1; O2 utilization, nmol min−1 mg protein−1; RCR (respiratory control ratio), unitless; Membrane potential (JC-1 fluorescence ratio), unitless; ATP production, nmol min−1 mg protein−1; Hepatic ATP, nmol mg tissue−1.
Data are presented as mean values ± S.E. (n = 6). A t-test was used to evaluate data.
P<0.05 versus vehicle-treated mice.