Table 2.

Direct effect of TCDD on the function of liver mitochondria from untreated mice.

	pmol TCDD/mg mitochondrial protein
Parameter	0	100	1000
state 4 respiration	82 ± 15	146 ± 12*	48 ± 13*
state 3 respiration	303 ± 25	572 ± 41*	187 ± 21*
RCR	3.70 ± 0.4	3.92 ± 0.5	3.9 ± 0.4
ATP production	436 ± 38	381 ± 34	114 ± 13*
ATP/O ratio	1.44 ± 0.16	0.67 ± 0.08*	0.61 ± 0.09*

Mitochondria were prepared from C57BL/6J untreated mice. To 0.6 ml KCl-RB containing 0.2 mg mitochondrial protein was added 2 μl DMSO or TCDD in DMSO at a concentration of 10 or 100 μM (to produce final concentrations of 0, 100, or 1000 pmol TCDD/mg mitochondrial protein, respectively). The units are: Respiratory rates, nmol min⁻¹ mg protein⁻¹; ATP production, nmol min⁻¹ mg protein⁻¹.

Data are presented as mean values ± S.E. (n = 6). Data were analyzed by a one-way ANOVA, followed by the Student-Newman-Kuels test for pair-wise comparison of means.

^*P<0.05 versus vehicle-treated mice.