Table 2.
pmol TCDD/mg mitochondrial protein | |||
---|---|---|---|
Parameter | 0 | 100 | 1000 |
state 4 respiration | 82 ± 15 | 146 ± 12* | 48 ± 13* |
state 3 respiration | 303 ± 25 | 572 ± 41* | 187 ± 21* |
RCR | 3.70 ± 0.4 | 3.92 ± 0.5 | 3.9 ± 0.4 |
ATP production | 436 ± 38 | 381 ± 34 | 114 ± 13* |
ATP/O ratio | 1.44 ± 0.16 | 0.67 ± 0.08* | 0.61 ± 0.09* |
Mitochondria were prepared from C57BL/6J untreated mice. To 0.6 ml KCl-RB containing 0.2 mg mitochondrial protein was added 2 μl DMSO or TCDD in DMSO at a concentration of 10 or 100 μM (to produce final concentrations of 0, 100, or 1000 pmol TCDD/mg mitochondrial protein, respectively). The units are: Respiratory rates, nmol min−1 mg protein−1; ATP production, nmol min−1 mg protein−1.
Data are presented as mean values ± S.E. (n = 6). Data were analyzed by a one-way ANOVA, followed by the Student-Newman-Kuels test for pair-wise comparison of means.
P<0.05 versus vehicle-treated mice.