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. Author manuscript; available in PMC: 2007 Jan 29.

Published in final edited form as: Bioorg Chem. 2006 Oct 27;34(6):362–379. doi: 10.1016/j.bioorg.2006.09.001

Inhibition of wild-type E1h and E1h αH63A by AcPhi and AcMPhi. Symbols are: wild-type E1h inhibited by AcPhi () and AcMPhi (); αH63A E1h inhibited by AcPhi () and AcMPhi (). E1h (6.49 μM) or its αH63A variant was incubated in 50 mM KH₂PO₄ (pH 7.0), 2 mM MgCl₂, 0.2 mM ThDP and different concentrations of AcPhi or AcMPhi for 30 min. Aliquots were taken to determine the E1-specific activity according to the DCPIP assay as described in Methods. 100% of the activity was determined in the absence of inhibitors in the incubation mixture and corresponds to 231 mU/mg of E1 protein. Data points are means ± SE (n = 3). Inset shows inhibition of the wild-type E1h by AcPhi () and AcMPhi () at lower concentrations.

Inline graphic — Inhibition of wild-type E1h and E1h αH63A by AcPhi and AcMPhi. Symbols are: wild-type E1h inhibited by AcPhi () and AcMPhi (); αH63A E1h inhibited by AcPhi () and AcMPhi (). E1h (6.49 μM) or its αH63A variant was incubated in 50 mM KH₂PO₄ (pH 7.0), 2 mM MgCl₂, 0.2 mM ThDP and different concentrations of AcPhi or AcMPhi for 30 min. Aliquots were taken to determine the E1-specific activity according to the DCPIP assay as described in Methods. 100% of the activity was determined in the absence of inhibitors in the incubation mixture and corresponds to 231 mU/mg of E1 protein. Data points are means ± SE (n = 3). Inset shows inhibition of the wild-type E1h by AcPhi () and AcMPhi () at lower concentrations.