Table 2.
Success rate and structural characteristics for RACE-targeted intergenic genes.
| Target Statistics | Twinscan | EuGene | Combined |
| Number targeted | 726 | 623 | 1071* |
| Mean CDS length | 456 bp | 354 bp | - |
| Median CDS length | 357 bp | 264 bp | - |
| Average # of exons | 2.0 | 1.4 | - |
| % single exon predictions | 423 (58%) | 452 (73%) | - |
| Experimental Statistics | Twinscan | EuGene | Combined |
| Number successful† | 304 (42%) | 257 (41%) | 378 (35%) |
| Mean CDS length | 445 bp | 452 bp | 441 bp |
| Median CDS length | 339 bp | 330 bp | 321 bp |
| Average # of exons | 1.8 | 1.7 | 1.7 |
* Primer pairs were designed for a total of 1071 total loci. 623 pairs of primers were compatible with a EuGene prediction, and 726 pairs of primers were compatible with a Twinscan prediction. 278 primer pairs were compatible with both a Twinscan and a EuGene prediction.
† For Twinscan and EuGene, success rates are defined as the number of FL sequences obtained that overlap a Twinscan or EuGene prediction, as compared to the number targeted. For the Combined category, the success rate represents the total number of loci verified with respect to the total number targeted.