TABLE 2.
Stern Volmer constants (Ksv) of acrylamide accessibility of wt and mutant GluR2 S1S2 tryptophan residues
| Apo (M−1) | PS (M−1) | PregaS (M−1) | |
|---|---|---|---|
| wt | 6.29 ± 0.22 | 6.65 ± 0.19 | 6.67 ± 0.54 |
| W460F | 5.44 ± 0.26 | 5.84 ± 0.28 | 5.28 ± 0.17 |
| W671F | 5.80 ± 0.15 | 6.31 ± 0.29 | 5.92 ± 0.12 |
| W766/767F | 6.36 ± 0.17 | 4.82 ± 0.25 | 4.88 ± 0.19 |
Acrylamide ranging in concentration from 39 to 280 mM was added to 5.2 μM GluR2 S1S2 mixed with ligand that was at a final concentration of 308 μM PS or PregaS, and the fluorescence emission at 340 nm was probed. Data was analyzed using the Stern-Volmer equation F0/F = 1 + KSV[Q]. Error is reported at the 95% confidence level.