TABLE 5.
Maximum wavelength of emission of the apo and ligand bound NMDA NR1-1b S1S2 domain
| Ligand | Maximum wavelength (nm) |
|---|---|
| Apo | 326 ± 3 |
| Spermidine | 325 ± 3 |
| Spermine | 324 ± 2 |
| Zinc acetate | 334 ± 2 |
The fluorescence emission at 325 nm of 3.75 μM NMDA NR1-1b S1S2 in buffer 3 was probed. Ligands were used in the following final concentrations: 75 mM zinc acetate, 2 mM spermidine, and 1 mM spermine.