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Effect of changing excitation wavelength on the wavelength of maximum emission of (A) N-ter and (B) Lys-7 NBD-melittin analogs bound to vesicles containing DOPC (○), DOPC/60%DOPG (▵), and DOPC/20%cholesterol (▪). The lines joining the data points are provided merely as viewing guides. The ratio of melittin/lipid was 1:100 (mol/mol) and the concentration of melittin was 4.27 μM in all cases. See Materials and Methods for other details.
