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Representative data for Stern-Volmer analysis of Co2+ quenching of the N-ter (○) and Lys-7 (•) NBD-melittin analogs bound to DOPC vesicles. Fo is the fluorescence in the absence of quencher, F is the corrected fluorescence in the presence of quencher. The excitation wavelength was 465 nm and emission was monitored at 530 nm. See Materials and Methods and Table 4 for other details.
