TABLE 2.
Fluorescence lifetimes of NBD-melittin analogs in membranes of varying lipid composition
| N-ter NBD-melittin*
| |||||
|---|---|---|---|---|---|
| Membrane | α1 | τ1 (ns) | α2 | τ2 (ns) | 〈τ〉 (ns) |
| DOPC | 0.26 | 4.69 | 0.74 | 10.26 | 9.49 (±0.03) |
| DOPC/60% DOPG | 0.68 | 5.54 | 0.32 | 10.73 | 8.01 (±0.11) |
| DOPC/20% chol | 0.67 | 5.82 | 0.33 | 10.15 | 7.82 (±0.24) |
| DOPC/40% chol | 0.69 | 5.70 | 0.31 | 10.06 | 7.63 (±0.21) |
| Lys-7 NBD-melittin
| |||||
| Membrane | α1 | τ1 (ns) | α2 | τ2 (ns) | 〈τ〉 (ns) |
| DOPC | 0.59 | 4.02 | 0.41 | 8.83 | 6.93 (±0.06) |
| DOPC/60% DOPG | 0.74 | 3.93 | 0.26 | 9.25 | 6.34 (±0.02) |
| DOPC/20% chol | 0.74 | 3.88 | 0.26 | 8.70 | 6.00 (±0.08) |
| DOPC/40% chol | 0.73 | 3.50 | 0.27 | 8.19 | 5.68 (±0.03) |
*
The excitation wavelength was 465 nm; emission was monitored at 525 and 530 nm for N-ter and Lys-7 NBD-melittin analogs, respectively. The values in parentheses represent the mean ± SE of at least three independent measurements. The number of photons collected at the peak channel was 10,000. See Table 1 and Materials and Methods for other details.