TABLE 3.

Fluorescence polarization of NBD-melittin analogs in membranes of varying lipid composition

	Fluorescence polarization		Apparent rotational correlation time (τc)* (ns)
Membrane	N-ter	Lys-7	N-ter	Lys-7
DOPC	0.278 ± 0.005	0.296 ± 0.003	13.0	10.9
DOPC/60% DOPG	0.213 ± 0.004	0.246 ± 0.001	6.1	6.4
DOPC/20% chol	0.302 ± 0.004	0.294 ± 0.002	13.4	9.5

^*The excitation wavelength was 465 nm; emission was monitored at 525 and 530 nm for N-ter and Lys-7 NBD-melittin analogs, respectively. The polarization values represent mean ± SE of at least three independent measurements. All other conditions are as in Table 1. See Materials and Methods for other details.