TABLE 1.

Electrophysiological characterization of N15C MscL mutant under different oxidative conditions

Tension sensitivity			Kinetics (ms)
Strain/Reagent	Threshold MscL/MscS	Amplitude (pA)	τ2	τ3
Wild-type	1.55 ± 0.06	81.3 ± 0.9	9.5 ± 3.3	28.3 ± 9.5
N15C/H2O2	1.40 ± 0.05*	82.8 ± 0.8	3.8 ± 0.9†	23.8 ± 5.7
N15C/DTT	1.73 ± 0.08§	83.3 ± 0.9	1.0 ± 0.1†‡	5.5 ± 1.1†‡

Tension sensitivity was derived using MscS as an internal control as described in Materials and Methods. Values are the mean ± SE from at least 10 patches that were derived from at least two independent spheroplast preparations. Kinetics were fit assuming three time constants; the shortest (τ 1) is below resolution (<1 ms). Kinetic values were derived from at least 1000 events and two independent spheroplast preparations.

^*p ≤ 0.001, by two-tailed, unpaired Student's t test against wild-type MscL.

^†p ≤ 0.0001, by two-tailed, unpaired Student's t test against wild-type MscL.

^‡p ≤ 0.05, by two-tailed, unpaired Student's t test against H₂O₂ treated.

^§p ≤ 0.001, by two-tailed, unpaired Student's t test against H₂O₂ treated.