TABLE 2.

Stability parameters of SARS-CoV full-length and C-terminal truncated main proteases

Protease	ΔGN-I (kcal mol−1)	mN-I (kcal mol−1 M−1)	[GdnCl]0.5,N-I (M)	ΔGI-U (kcal mol−1)	mI-U (kcal mol−1 M−1)	[GdnCl]0.5,I-U (M)
3CLpro*	1.22 ± 0.16	1.15 ± 0.11	1.1 ± 0.2	10.88 ± 0.27	2.70 ± 0.07	4.0 ± 0.1
W31†	1.39 ± 0.46	0.91 ± 0.22	1.5 ± 0.6	–	–	–
W207/W218†	–	–	–	10.25 ± 0.47	2.49 ± 0.12	4.1 ± 0.3
3CLI+II†	7.27 ± 0.35	4.94 ± 0.23	1.5 ± 0.1

^*The unfolding data were fitted to a three-state unfolding model according to Eq. 8.

^†The unfolding data were fitted to a two-state unfolding model according to Eq. 7.