Fig. 4.

Microarray results confirmed by in situ hybridization histochemistry (A) and functional analysis of thalamus-enriched genes (B). In situ hybridization and functional annotation validate thalamic nucleus-specific gene expression. (A) Images from film autoradiograms show differential expression of CBLN1 (Upper) and PCP4 (Lower) in monkey dorsal thalamus, corroborating the microarray results (Right). (B) The Ingenuity Pathways Analysis program enabled biological/functional annotations of candidate genes identified by the Drawn Gene Function in GeneSpring analysis. The top five functions are compared across all regions. Abbreviations are as in Fig. 2.