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The curves were made using Origin 6.0 software. Sedimentation velocity measurements of (propyl-PEG5K)6-Hb (□), (propyl-PEG5K)6-αα-fumaryl Hb (○), HbA (■) and αα-fumaryl Hb (●) were conducted in a Beckman XL-I analytical ultracentrifuge in PBS, pH 7.4, at 25 °C and 55000 rev./min. Boundary movement was followed at 405 nm using the centrifuge's absorption optics.
