Figure 4. Specific binding of a PDTC-stimulated transcription factor complex to Cp-APRE.

EMSAs were carried out to determine the Cp-APRE complexes. (A) HepG2 cells were treated for 2 h with increasing concentrations of PDTC. S denotes treatment with 10% fetal bovine serum. (B) Hep3B cells were treated with 100 μM PDTC for different time periods. In both (A) and (B), after the treatment, nuclear extracts were prepared and incubated with a ³²P-labelled double-stranded 24-mer probe containing the Cp-APRE. Probe-bound complexes were resolved by 5% nondenaturing PAGE and visualized by autoradiography.