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. 2007 Jan 25;402(Pt 1):135–141. doi: 10.1042/BJ20060963

Figure 7. The extracellular copper chelator BCS blocks PDTC-induced activation of AP-1.

Figure 7

(A) Hep3B cells were pretreated with BCS (50 μM) 30 min before PDTC (100 μM) treatment. Nuclear extracts were prepared after 2 h of PDTC treatment and mixed with a radiolabelled Cp-APRE probe to perform EMSA. (B) HepG2 cells were transiently transfected with a chimaeric construct containing the proximal 3701 bp of the Cp gene along with β-galactosidase. After recovery, the cells were pretreated with 50 μM of BCS, 30 min before PDTC (100 μM) treatment. After 16 h, luciferase activity was determined by chemiluminescence and normalized for β-galactosidase activity. Means±S.D. n=3, *P<0.05, **P<0.01 and ***P<0.001.