Figure 2. Time course of bZIP transcription factor accumulation during amino acid starvation in rat C6 glioma cells.
(A) C6 glioma cells were starved of amino acids for the indicated times and protein levels in nuclear extracts were determined by Western blotting as described in the Materials and methods section. The arrow indicates the location of the CHOP protein. (B) Protein levels in (A) were quantified and normalized to the level in cells prior to starvation. (C) Protein levels in whole cell extracts were analysed by Western blotting.
