Skip to main content
. 2007 Jan 25;402(Pt 1):25–34. doi: 10.1042/BJ20061170

Figure 2. Immunofluorescence microscopy of endogenous Ns in E15 primary mouse cortical cultures.

Figure 2

Ns (green) is detected in a representative neuron (red) and forms a punctate pattern similar to that observed in human neurons in Figure 1. (A) Polyclonal anti-Ns antibodies; (B) DAPI nuclear stain (blue); (C) a phase contrast image of the same cell; (D) a monoclonal antibody of the neuronal specific marker NeuN; (E) a three-colour merged image of Ns, DAPI and NeuN; (F) a merged image of (C) and (E). The secondary antibodies used were anti-rabbit Alexa Fluor® 488 for Ns and anti-mouse RRX for NeuN, and the original magnification was with a ×100 oil-immersion objective lens. Scale bar, 5 μm.