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. 2007 Jan 25;402(Pt 1):25–34. doi: 10.1042/BJ20061170

Figure 6. The Ns C-terminus is exposed on the surface of the molecule.

Figure 6

Conditioned media from wtNs (lanes 1, 3 and 5) or Δ13Ns (lanes 2, 4 and 6) was immunoprecipitated with either polyclonal anti-Ns (α-Ns; lanes 3 and 4) or with anti-Ns15 antibodies (α-Ns15; lanes 5 and 6), which detects only the Ns C-terminus, followed by immunoblotting with biotinylated rabbit anti-Ns. Lanes 1 and 2 are starting material not subjected to immunoprecipitation. Notice that anti-Ns15 antibody precipitated wtNs (lane 5) but not Δ13Ns (lane 6). Arrows show the position of a 50 kDa marker.