Figure 9. Secretion of the PAI-Ns chimaeric protein is regulated.

Pulse–chase metabolic labelling was performed in Att-20 cell lines as in Figure 8 with (+) or without (−) 2 mM BaCl₂ during the 1 h chase period. Cells expressing wtNs, wtPAI-1 and two independent clones of the PAI-Ns chimaera were examined. Following immunoprecipitation with either polyclonal anti-PAI-1 (wtPAI-1 and PAI-Ns) or anti-Ns (wtNs) antibodies, samples were subjected to SDS/PAGE and STORM imager analysis. (A) Representative scan of the SDS/PAGE. (B) Quantification of the fold increase in Ns or PAI-1 secretion induced by barium stimulation. The data show the results of two independent experiments performed in triplicate for each protein. P<0.05 using Student's t test was obtained for stimulated secretion of wtNs and the two chimaera compared with the controls in the absence of barium. There was no significant increase in secretion with the wtPAI-1.