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. Author manuscript; available in PMC: 2007 Nov 9.
Published in final edited form as: Neuron. 2006 Nov 9;52(3):445–459. doi: 10.1016/j.neuron.2006.08.033

Figure 3.

Figure 3

Arc is Recruited with Endophilin and Dynamin to Vesicles in HeLa Cells(A) Arc expressed alone has a cytoplasmic and nuclear distribution in HeLa cells, with some enrichment at the plasma membrane. En3-CT [Endophilin 3(172-347)] alone has a cytoplasmic distribution with enrichment at the plasma membrane. Dyn2-CT [Dynamin 2(503-871)] alone localizes to vesicular structures near the plasma membrane. When Arc and En3-CT are expressed together, vesicular structures form that contain both proteins. Arc and Dyn2-CT co-expression results in a redistribution of Arc to dynamin 2 containing vesicular structures. When Arc, Dyn2-CT, and En3-FL (endophilin 3 full length) are co-expressed, all three proteins colocalize in vesicular structures.

(B) Arc and full-length endophilin 3 have no effect on Alexa-555 conjugated transferrin uptake when expressed alone (a and c). Arc and myc antibodies were used to stain Arc and endophilins respectively. (b) En3-CT inhibits transferrin uptake. (d) Arc and En3-CT co-expression results in endosomes that internalize transferrin. (Scale bars represent 30 μm)