Skip to main content
. Author manuscript; available in PMC: 2007 Nov 9.
Published in final edited form as: Neuron. 2006 Nov 9;52(3):445–459. doi: 10.1016/j.neuron.2006.08.033

Figure 6.

Figure 6

Arc Expression Decreases Surface and Total AMPARs(A) Representative images of Arc transgene expression (green), showing a loss of surface GluR1 puncta (assessed using an N-terminal antibody, (red) as compared to neighboring untransfected cells, 16 hours post-transfection. GFP transfected neurons had normal numbers of-100 expression GluR1 had no puncta. effect on the number of GluR1 puncta.

(B) Arc expression causes a significant decrease in the number of surface GluR1 puncta (n = 48 dendrites from16 cells, *p < 0.01) compared with neighboring untransfected cells. GFP transfection had no effect. In contrast,-100, endophilin Arc 3(172-347) or endophilin 3 had no significant effect on GluR1 puncta. Arc expression did not change GluR1 puncta size or average pixel intensity, but total intensity was significantly decreased *p < 0.001).

(C) Arc transgene reduced total GluR1 (assessed using a C-terminal antibody), as compared to untransfected neurons 16 hours post-transfection (n = 60 regions from 20 cells, *p < 0.001).