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. 2007 Jan 30;104(6):1841–1846. doi: 10.1073/pnas.0605562104

Fig. 3.

Fig. 3.

Depletion of BLIMP1 elevates p53 and induces the expression of p53 target genes. Lysates were immunoblotted with the indicated antibodies. RNA was isolated, reverse-transcribed, subjected to qPCR, and normalized with GAPDH mRNA. (A) Immunoblots of total lysates of HCT116 cells transfected with control siRNA or BLIMP1 siRNAs. (B) RT-PCR analysis of mRNA levels in HCT116 p53+/+ and p53−/− cells transfected with BLIMP1 siRNAs or control siRNA. (C) Immunoblots of total lysates of IMR90 cells transfected with control siRNA or BLIMP1 siRNAs. (D) RT-PCR analysis of p53 mRNA levels in HCT116 and IMR90 cells transfected with BLIMP1 siRNA-1 or control siRNA.