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. 2007 Feb 14;2(2):e211. doi: 10.1371/journal.pone.0000211

Figure 6. In vitro tellurite reduction generates superoxide in E. coli.

Figure 6

Superoxide generation was evaluated using an in vitro tellurite reduction assay previously developed in our laboratory [34]. The system makes use of the O2 − specific reactive compound WST-1. WST-1 reduction was determined in the presence of catalase and NADPH (Cat); catalase, tellurite and NADPH (Cat/Te); catalase, tellurite, NADPH and purified superoxide dismutase (Cat/Te/SOD); catalase, tellurite, NADPH and β-amylase (Cat/Te/amylase).