Table 2.
Biochemical properties of recombinant wild-type and mutant LHCII and CP29
| Recombinant protein | Binding site affected | Reconstitution efficiency, % | Chl a+b/ polypeptide | Xanthophylls | Chl/carotenoid ratio | Chl-a/b ratio | Chl-a polypeptide, no. | Chl-b polypeptide, no. | Stability at 60°C t1/2, min |
|---|---|---|---|---|---|---|---|---|---|
| CP29 | |||||||||
| Wild type | — | 100 | 8 | 2 | 4.00 | 3.00 | 6 | 2 | 4.2 |
| H245L | B3 | 94 | 7 | 2 | 3.50 | 4.35 | 5.7 | 1.3 | 2.0 |
| H216F | A2 | 75 | 7 | 2 | 3.10 | 2.53 | 5 | 2 | 4.0 |
| H114F | A5 | 53 | 7 | 2 | 3.47 | 2.42 | 5 | 2 | 0.5 |
| Q230L | A3 | 90 | 7 | 2 | 3.45 | 3.11 | 5.3 | 1.7 | 1.7 |
| R218L | A4 | 0 | |||||||
| E111V | A4 | 0 | |||||||
| E111V/R218L | A4 | 45 | 7 | 2 | 3.85 | 2.50 | 5 | 2 | 3.3 |
| E166V | B6 | 45 | 6 | 1 | 6.12 | 5.02 | 5 | 1 | 0.4 |
| E166Q | B6 | 90 | 8 | 2 | 4.00 | 2.34 | 5.6 | 2.4 | 5.2 |
| R177L | B5 | 0 | |||||||
| E174V | B5 | 55 | 7 | 1.5 | 4.75 | 3.91 | 5.6 | 1.4 | 0.5 |
| R177L/E174V | B5 | 0 | |||||||
| E213V | A1 | 0 | |||||||
| R116L | A1 | 0 | |||||||
| R116L/E213V | A1 | 0 | |||||||
| LHCII | |||||||||
| Wild type | — | 100 | 12 | 3 | 4.02 | 1.4 | 7 | 5 | ND |
| Q131E | B6 | 94 | 12 | 3 | 4.05 | 1.65 | 7.4 | 4.6 | ND |
Standard deviations: Chl-a/b ratio, 0.04 (8–12 independent measurements; Chl/xanthophyll ratio, 0.2 (six independent measurements). Reconstitution efficiency represents the yield in CP with respect to wild type. In the case of LHCII, 25% of the wild-type apoprotein used in reconstitution was recovered as CP. ND, not determined.