Figure 4.

Distinct functional properties of naïve CD4 T cells stimulated by ICAM ligands in the presence of CD3/CD28. (A) Intracellular expression of BCL-2 following stimulation of naïve CD4+ T cells with CD3, CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28. Mean values for fluorescent intensity are indicated by the histogram. Assay was performed in triplicates from four donors. (B) Frequency of IFNγ producing CD4+T cells after 7 days of stimulation with CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28. Assay displays standard deviation from triplicate measurements and was repeated in T cells from 4 different donors. (C) Secreted production of TGFβ1 after 48 hours of stimulation. Graph displays standard deviation from triplicate experiments from two different donors. tTest calculations were performed and indicated in the table insert, with p < 0.05 being significant