Figure 1.
Effect of protein synthesis inhibitors on protein prenylation. (A) Protein prenylation in spinach cotyledons. Cotyledons were labeled with [3H]mevalonate. After the labeling period, the samples were homogenized, lipid-extracted, and analyzed by SDS/PAGE followed by fluorography. C, control; CH, prenylation in the presence of 150 μM CH, CAP, prenylation in the presence of 150 μM CAP. (B) Prenylated proteins in isolated, intact chloroplasts. Labeling as in A. (C) Prenylated proteins in: lane 1, neuroblastoma N2a cells; lane 2, rat kidney.