Figure 2.
HPLC analysis of covalently bound isoprenoids. Cotyledons were labeled with [3H]mevalonate in the presence of CAP (150 μM). After labeling, proteins were lipid-extracted and subjected to hydrolysis. The released isoprenoids were analyzed by RP- HPLC, and the resulting chromatograms are shown. (A) Methyl iodide hydrolysis. (Inset) Isoprenoids released from cotyledon proteins labeled in the presence of CH (150 μM). (B) Alkaline hydrolysis of proteins labeled in the presence of CH. The elution of farnesol (F), geranylgeraniol (GG), and phytol (PH) are indicated.