Figure 3.

Identification of phytol by MS. Lipid-extracted thylakoid proteins from 1 kg of spinach were subjected to alkaline hydrolysis followed by RP-HPLC. The eluate corresponding to the elusion time of phytol standard was collected, the solvents were evaporated, and the residue was derivatized to the acetate form. The resulting product was analyzed on GC-MS. (A, Upper) GC chromatogram of phytyl acetate standard. (A, Lower) The fragmentation pattern of phytyl acetate (elution time 36.2 min). (B, Upper) GC chromatogram of the spinach isoprenoid. (B, Lower) The fragmentation pattern of the compound eluting at 36.24 min.