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. 1999 Aug 31;96(18):10080–10085. doi: 10.1073/pnas.96.18.10080

Figure 4.

Figure 4

Expression of antisense PP2A-B55 inhibits induction of apoptosis by E4orf4. (A) Plasmids expressing HA-tagged PP2A-B55 and E4orf4 were cotransfected into H1299 cells with the empty CMV vector (CMV), or with a plasmid expressing PP2A-B55 in the sense (B55), or in the antisense (B55-AS) orientation. Expression of HA-PP2A-B55 was detected by a Western blot. (B) H1299 cells were transfected as in A, but the transfection mix contained a plasmid expressing green fluorescent protein and not the HA-tagged PP2A-B55. pCMV-p53 was used in some experiments instead of E4orf4. The number of apoptotic cells was determined 48 hr posttransfection by counting 4′,6-diamidino-2-phenylindole dihydrochloride-stained apoptotic nuclei in the transfected cell population. One hundred transfected cells were counted in each experiment. Apoptosis induced by E4orf4 or p53 was defined as 100%. The average of four experiments is shown.