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. 1998;6(5):214–219. doi: 10.1002/(SICI)1098-0997(1998)6:5<214::AID-IDOG5>3.0.CO;2-I

Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision.

A L Distéfano 1, M A Picconi 1, L V Alonio 1, D Dalbert 1, J Mural 1, O Bartt 1, G Bazán 1, G Cervantes 1, M Lizano 1, A G Carrancá 1, A Teyssié 1
PMCID: PMC1784810  PMID: 9894176

Abstract

OBJECTIVE: The aim of this study was to identify human papillomavirus (HPV) in cervical intraepithelial neoplasia (CIN) lesions and to evaluate the persistence of viral DNA after diathermic large loop excision (DLLE) treatment. STUDY DESIGN: Biopsies from 36 patients with low- and high-grade CIN lesions were studied before and after DLLE treatment looking for HPV sequences. DNA was extracted to perform a radioactive polymerase chain reaction (PCR) using GP 5,6 generic primers. PCR products were analyzed by the single-stranded conformational polymorphism (SSCP) which is a simultaneous detection and typing method. Dot-blot hybridization with generic and type-specific biotinylated oligonucleotide probes was applied in some cases. RESULTS: HPV DNA was found in all pretreatment samples, and the viral type was identified in 80% of them, HPV 16 being the most prevalent. The viral type coincided with that detected in the first biopsy in all except one case. Seventy five percent of the patients (27 cases) were negative for CIN at follow up, but 50% of them remained HPV DNA positive. CONCLUSION: DLLE treatment was effective in removing the CIN lesion but not the HPV. This fact points out the need to asses the presence of HPV in DNA during the follow-up, since viral persistence has been considered a high risk factor for recurrence and/or malignant transformation.

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Selected References

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  1. Bauer H. M., Hildesheim A., Schiffman M. H., Glass A. G., Rush B. B., Scott D. R., Cadell D. M., Kurman R. J., Manos M. M. Determinants of genital human papillomavirus infection in low-risk women in Portland, Oregon. Sex Transm Dis. 1993 Sep-Oct;20(5):274–278. doi: 10.1097/00007435-199309000-00007. [DOI] [PubMed] [Google Scholar]
  2. Bauer H. M., Ting Y., Greer C. E., Chambers J. C., Tashiro C. J., Chimera J., Reingold A., Manos M. M. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA. 1991 Jan 23;265(4):472–477. [PubMed] [Google Scholar]
  3. Bosch F. X., Manos M. M., Muñoz N., Sherman M., Jansen A. M., Peto J., Schiffman M. H., Moreno V., Kurman R., Shah K. V. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995 Jun 7;87(11):796–802. doi: 10.1093/jnci/87.11.796. [DOI] [PubMed] [Google Scholar]
  4. Brisson J., Bairati I., Morin C., Fortier M., Bouchard C., Christen A., Bernard P., Roy M., Meisels A. Determinants of persistent detection of human papillomavirus DNA in the uterine cervix. J Infect Dis. 1996 Apr;173(4):794–799. doi: 10.1093/infdis/173.4.794. [DOI] [PubMed] [Google Scholar]
  5. Chua K. L., Hjerpe A. Persistence of human papillomavirus (HPV) infections preceding cervical carcinoma. Cancer. 1996 Jan 1;77(1):121–127. doi: 10.1002/(SICI)1097-0142(19960101)77:1<121::AID-CNCR20>3.0.CO;2-6. [DOI] [PubMed] [Google Scholar]
  6. Cox J. T., Lorincz A. T., Schiffman M. H., Sherman M. E., Cullen A., Kurman R. J. Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance. Am J Obstet Gynecol. 1995 Mar;172(3):946–954. doi: 10.1016/0002-9378(95)90026-8. [DOI] [PubMed] [Google Scholar]
  7. Evander M., Edlund K., Bodén E., Gustafsson A., Jonsson M., Karlsson R., Rylander E., Wadell G. Comparison of a one-step and a two-step polymerase chain reaction with degenerate general primers in a population-based study of human papillomavirus infection in young Swedish women. J Clin Microbiol. 1992 Apr;30(4):987–992. doi: 10.1128/jcm.30.4.987-992.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lizano M., Berumen J., Guido M. C., Casas L., García-Carrancá A. Association between human papillomavirus type 18 variants and histopathology of cervical cancer. J Natl Cancer Inst. 1997 Aug 20;89(16):1227–1231. doi: 10.1093/jnci/89.16.1227. [DOI] [PubMed] [Google Scholar]
  9. Melki R., Khoury B., Catalan F. Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences. J Med Virol. 1988 Oct;26(2):137–143. doi: 10.1002/jmv.1890260205. [DOI] [PubMed] [Google Scholar]
  10. Minucci D., Cinel A., Insacco E. Diathermic loop treatment for CIN and HPV lesions. A follow-up of 130 cases. Eur J Gynaecol Oncol. 1991;12(5):385–393. [PubMed] [Google Scholar]
  11. Muñoz N., Bosch F. X. HPV and cervical neoplasia: review of case-control and cohort studies. IARC Sci Publ. 1992;(119):251–261. [PubMed] [Google Scholar]
  12. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766–2770. doi: 10.1073/pnas.86.8.2766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reid R., Scalzi P. Genital warts and cervical cancer. VII. An improved colposcopic index for differentiating benign papillomaviral infections from high-grade cervical intraepithelial neoplasia. Am J Obstet Gynecol. 1985 Nov 15;153(6):611–618. doi: 10.1016/s0002-9378(85)80244-1. [DOI] [PubMed] [Google Scholar]
  14. Schiffman M. H., Bauer H. M., Hoover R. N., Glass A. G., Cadell D. M., Rush B. B., Scott D. R., Sherman M. E., Kurman R. J., Wacholder S. Epidemiologic evidence showing that human papillomavirus infection causes most cervical intraepithelial neoplasia. J Natl Cancer Inst. 1993 Jun 16;85(12):958–964. doi: 10.1093/jnci/85.12.958. [DOI] [PubMed] [Google Scholar]
  15. Schiffman M. H. New epidemiology of human papillomavirus infection and cervical neoplasia. J Natl Cancer Inst. 1995 Sep 20;87(18):1345–1347. doi: 10.1093/jnci/87.18.1345. [DOI] [PubMed] [Google Scholar]
  16. Snijders P. J., van den Brule A. J., Schrijnemakers H. F., Snow G., Meijer C. J., Walboomers J. M. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol. 1990 Jan;71(Pt 1):173–181. doi: 10.1099/0022-1317-71-1-173. [DOI] [PubMed] [Google Scholar]
  17. Van Den Brule A. J., Walboomers J. M., Du Maine M., Kenemans P., Meijer C. J. Difference in prevalence of human papillomavirus genotypes in cytomorphologically normal cervical smears is associated with a history of cervical intraepithelial neoplasia. Int J Cancer. 1991 May 30;48(3):404–408. doi: 10.1002/ijc.2910480317. [DOI] [PubMed] [Google Scholar]
  18. Villa L. L. Human papillomaviruses and cervical cancer. Adv Cancer Res. 1997;71:321–341. doi: 10.1016/s0065-230x(08)60102-5. [DOI] [PubMed] [Google Scholar]

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