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. 2006 Sep 5;109(1):85–92. doi: 10.1182/blood-2006-05-020289

Table 1.

Effect of recombinant cytokines and HS5 CM on MMP-9 gene expression and protein secretion in CD14+ cells

Concentration, ng/mL MMP-9 gene expression MMP-9 protein secretion
HS5 CM* 500 8756
MCP-1/CCL2
    0.05 236 153
    0.5 409 1089
    5 263 136
IL-1β
    0.005 211 95
    0.05 312 155
    0.5 356 576
IL-6
    0.5 146 119
    5 244 124
    50 206 121
    500 161 205
M-CSF
    0.05 180 163
    0.5 152 287
G-CSF, 10 193 ND
GM-CSF, 100 59 ND
MGSA/CXCL1, 100 111 ND
IL-8/CXCL8, 10 125 ND
SDF-1a/CXCL12, 100 125 ND

Data are represented as percent control. Control values were derived from CD14 cells cultured for 5 days in control media consisting of 10% FCS in RPMI 1640 medium.

ND indicates not determined.

*

HS5 CM (conditioned medium) was diluted with an equal volume of fresh medium for use in culturing CD14+ cells. Concentrations of the cytokines in HS5 CM were: IL-1β, 0.25 ng/mL; MCP-1, 0.5 ng/mL; M-CSF, 0.25 ng/mL; and IL-6, 250 ng/mL. CD14 cells were harvested for RNA and supernatants collected for protein detection after 5 days of culture.

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