Figure 4.

Multiple Rap1 binding suppresses Mre11 and Cdc13 recruitment at long TG tracts. (A) Analysis by ChIP of the binding of Mre11-Myc and Cdc13-Myc after galactose induction of a DSB in wild-type and rap1-17 strains. (B) Southern blots monitoring cleavage at the HO site in a rap1-17 strain. (INT) The internal loading control; (U) an uncut fragment; (C) the fragment resulting from “U” after induction of the HO cut. (C) Analysis by ChIP of the binding of Mre11-Myc and Cdc13-Myc after galactose induction of a DSB adjacent to 16× and 4× wild-type and mutant Rap1 site arrays.