Figure 6.

In ste11^T82A mutants, the periodicity in expression of Ste11 and mfm2 is lost. An exponentially growing cdc25-22 ste11^T82A strain was shifted from 25°C to 35.5°C, and after 3.5 h of incubation, the culture was released at the permissive temperature. After the release, the levels of Ste11 protein and mfm2 transcript were followed by Western analysis (middle panel) and RT–PCR (bottom panel), respectively. (Top panel) The synchrony of the culture was followed by determining the percentage of cells in anaphase (open squares) and cells with a septum (closed squares).