Table 2.

Dissociation constants of Ste11 proteins

^aRecombinant Ste11 proteins were added in different concentrations to 1 nM of a TR-box-containing probe, and the samples were incubated until the binding reactions were at equilibrium. Free and bound DNA was separated by EMSA, and K_d was determined as the concentration of Ste11 protein required to bind 50% of the probe. Values represent means and standard deviations (SD) of three separate trials.

^bBinding is significantly different from that of wild-type Ste11 (P < 0.05; tested by Student’s t-test).