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Cytoplasmic localization of Dnmt2 rescues Dnmt2 morphant phenotypes. (A) Confocal images of GFP and TOPRO-3 fluorescence in ZF4 and RKO cells transfected with empty vector, or vectors containing tagged Dnmt2WT, NLS-Dnmt2, mNLS-Dnmt2, or NES-Dnmt2. (B) Whole-mount in situ of embryos at 80 hpf, stained for expression of l-fabp and vsx2. Columns depict embryos from control morphants, Dnmt2 morphants, or Dnmt2 morphants coinjected with NLS-Dnmt2, mNLS-Dnmt2, or NES-Dnmt2.
