Figure 3.

The structure of HEWL is color coded according to the magnitude of the effect of D1.3 binding on the stability constants per residue. In this figure, red denotes the region of HEWL in direct contact with D1.3. Yellow indicates the regions that are predicted to have enhanced stability constants, even though they are not in contact with the antibody. Blue denotes the regions predicted not to be affected by binding. The helices and β-domain have been labeled on the structure. The stability constants were calculated with the Core_Bind algorithm (15), and the figure was made with the program grasp (28).