Fig. 6.

Jjj1 stimulates the ATPase activity of Ssa1. (A) Ten OD₂₅₄ units of lysate from the Δssb1 Δ ssb2 (Δssb1/2) grown at 23°C were centrifuged through a 5–50% sucrose gradient, and the OD₂₅₄ was monitored. (B) Approximately equal numbers of cells of the indicated genotypes were diluted 10-fold and spotted on minimal media. Plates were incubated at 23°C. (C) Hsp70–ATP-[³²P] complexes were incubated in the presence of various concentrations of Jjj1, Jjj1_HPD→AAA, Ydj1, or Zuo1–Ssz1 heterodimer. The rates of hydrolysis of ATP were determined, and fold stimulation over the basal rate was plotted.