Fig. 2.

Cytoskeletal-like organization of the RNA degradosome components. (A) Differential interference contrast micrographs of strain AT1/pRNE1 [Δrne/P_lac-rne::yfp]. (B) Strain AT1/pRNE1 [Δrne/P_lac-rne::yfp] cell showing coiled structure of RNaseE-Yfp. (C–K) Immunofluorescence micrographs using anti-HA antibody (C and E–K) or purified anti-RhlB antibody (D). (C) Strain AT25 [rne::HA]. (D) Strain MC1000. (E) Strain AT18 [eno::HA]. (F) Strain AT19 [pnp::HA]. (G) Strain AT27 [rne^1–659::HA]. (H and I) Strain AT28 [rne^1–417::HA]. rne^1–417 cultures contain cells of variable length, including filamentous cells (see Results). (J) A22-treated AT25 [rne::HA] (see Results for details). (K) Strain AT41 [Δmin, rne::HA]. Because of the Min⁻ phenotype, the cells are predominantly short filaments. (Scale bars: 1 μm.)