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Differences in donor fluorescence decays within the unfolded state FRET peaks at 4 M GdmCl for protein L (A) and CspTm (B). The donor photons in the green and red curves are from bursts on the high-efficiency (green) and low-efficiency (red) side of the unfolded peak, respectively (see Insets). The donor fluorescence decays faster for bursts with higher 〈E〉.
