Fig. 6.

Regulation of thymocyte development by Notch/RBP-J signaling. It has been demonstrated that Notch/RBP-J signaling is required for intrathymic T cell development at three steps: (i) ETP production, (ii) γδΤ segregation, and (iii) DN3–DN4 transition. This work reveals that Mint negatively regulates Notch/RBP-J signaling at ETP production and DN3–DN4 transition. Furthermore, this work identified DN1–DN2 transition as a regulatory step controlled by Notch/RBP-J signaling, and it showed that Mint negatively regulates Notch/RBP-J signaling at this step. DN1 cells include ETP and recent immigrants from bone marrow which are represented by LSKⁱⁿ. Circulating LSK are shown by LSK^out.