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. 2007 Jan 24;104(5):1643–1648. doi: 10.1073/pnas.0610024104

Fig. 2.

Fig. 2.

Paracrine factors from Akt-MSCs mediate the survival signaling on cardiomyocytes. (a) Western blot analysis for Sfrp2 demonstrates presence of Sfrp2 protein in conditioned medium collected from Akt-MSCs or GFP-MSCs and inhibition of its accumulation in the medium in the presence of PI3-kinase inhibitor. (b) Relative reduction in mRNA levels of Sfrp2 in Akt-MSCs after knockdown of Sfrp2 with siRNA. (c) Effect of conditioned medium on apoptosis in ARVCs. Caspase activity of ARVCs after 24 h of hypoxia under different culture conditions (Ctr CM, control conditioned medium; Akt CM, Akt-conditioned medium; and Akt CM minus Sfrp2, Akt-conditioned medium after Sfrp2 knockdown) demonstrates reduction of caspase activity after Akt-conditioned medium treatment (Akt CM) and attenuation of this effect after treatment with Akt-conditioned medium after Sfrp2 knockdown (Akt CM minus Sfrp2).