Table 1A.
Flow cytometric analysis of 7-AAD negatively gated, viable Colo−/Colo+ and CX−/CX+ carcinoma sublines
Marker | Plasma membrane–positive cells, % ± SE* (mean fluorescence intensity) |
|||
---|---|---|---|---|
Colo− | Colo+ | CX− | CX+ | |
Hsp70 | 34 ± 5 (25 ± 5) | 73 ± 5† (29 ± 7) | 20 ± 6 (10 ± 4) | 90 ± 8† (18 ± 3) |
Bag-4 | 29 ± 11 (24 ± 2) | 78 ± 1† (30 ± 5)† | 42 ± 10 (8 ± 1) | 78 ± 2† (17 ± 1)† |
MHC I | 99 ± 1 | 98 ± 2 | 98 ± 1 | 98 ± 2 |
Data represent mean percentages of Hsp70 (cmHsp70.1), Bag 4 (IMG-152), and MHC I (W6/32) plasma membrane-positively stained cells of three independent experiments ± SE.
Significantly different from the corresponding negative cell type (P < 0.01).